MAGUS Lum V500 Fluorescence Inverted Microscope

The microscope is intended for studying specimens using the fluorescence microscopy technique in reflected light and using the brightfield and phase contrast techniques in transmitted light.
The fluorescence microscopy is based on the ability of some substances to glow when exposed to light of a certain part of the spectrum. The specimens are exposed to invisible short-wave ultraviolet light or violet blue and green light. The wavelength of the emitted light will be longer than the wavelength of the excitation light. The specimen glows with blue cyan green-yellow or red light respectively. Some specimens glow by themselves while others glow after treatment with fluorochromes.
The microscope is used for the examination of liquid precipitates cell colonies living cells tissue cultures and other samples in nutrient medium in glassware up to 55mm with a bottom thickness of 1.2mm. The microscope uses special objectives for working with such glassware. The illuminator stand is tiltable for use with Petri dishes or flasks up to 165mm. The microscope is suitable for immunochemical diagnostics and chromosome analysis as well as the detection of latent bacterial and viral infections.

Microscope head

Tube length – infinity (∞). When assembling the microscope the user can rotate the eyepiece tubes 180° and adjust eye relief to fit their height. There is a trinocular microscope head. The monitor is mounted in the vertical tube of the trinocular head and the digital camera – in the camera side port. The trinocular head has an 80:20 beam splitter and there is a 2-position beam splitter on the body (100:0/0:100).

Revolving nosepiece

The 6-objectives revolving nosepiece is mounted on a stand under the stage.

Objectives

Infinity plan achromatic objectives with long working distance for dishes with a bottom thickness of 1.2mm. Three objectives are intended for brightfield and fluorescence microscopy techniques and the other 3 objectives are for the phase contrast technique.

Focusing mechanism

Coaxial coarse and fine focusing knobs are located at the base of the microscope on both sides. The user can place their hands on the table and take a relaxed pose while observing. The focusing adjustment is smooth and effortless. On the right there is a coarse focusing lock knob for quick adjustments after changing the specimen. On the left there is a coarse tension adjustment ring for further adjustment.

Stage

There is a fixed stage with a mechanical attachment for moving the laboratory ware in two mutually perpendicular directions. The smooth and subtle movement of the object augments the accuracy of the study – no part of the specimen will be overlooked. The travel knob is located at the bottom and so the user will not strain their right hand. The microscope set includes four dish holders for dishes of different sizes.

Reflected light illuminator

The fluorescence excitation source is a 100W mercury lamp. It is extremely bright and has a wide spectrum of wavelengths. The mercury lamp has discrete peaks allowing you to work with many fluorochromes.
The reflected light illuminator contains four excitation filters: ultraviolet (UV) violet (V) blue (B) and green (G).
The mercury lamp is located in the lamphouse and can be centered in three planes. The heat radiation is conducted away from the lamphouse and therefore the lamp does not get too hot. The mount allows for safe and quick lamp replacement.

Condenser

Phase contrast turret condenser with four positions. There is a free slot for brightfield microscopy; the other three slots with phase annuli plates are used for working with objectives at magnifications of 10x 20x and 40x. The phase contrast rings can be centered. Fast and intuitive switching between microscopy techniques saves time and simplifies the researcher’s work.

Reflected light source

The reflected light illuminator has a 30W halogen bulb. Halogen bulbs emit light with a color temperature that allows for comfortable work. The 30W bulb is bright enough to observe with all the objectives that are used for brightfield and phase contrast microscopy techniques.

Köhler illumination in transmitted light

The Köhler illuminator improves the image quality of the observed sample: Each objective achieves maximum resolution. The field of view is illuminated evenly without darkening at the edges. The object of study is in sharp focus and the image artifacts are removed.

Accessories

There is a line of accessories designed specifically for this microscope.
Eyepieces that extend the magnification range of the microscope. The optional eyepieces help you maximize the potential of the objective that you use most often.
A digital camera that outputs the microscope image on a monitor and stores files along with software that takes real-time measurements of specimens.
A calibration slide for measuring objects that can be combined with an eyepiece with a scale or the camera software.

Key features:

• Study of specimens in laboratory ware emitting fluorescence when exposed to excitation light
• Microscopy techniques: fluorescence in reflected light brightfield and phase contrast in transmitted light
• Köhler illumination setup
• Tiltable stand for dishes up to 165mm
• Trinocular head with the option to adjust eye relief
• Two options for mounting a digital camera and monitor: vertical tube of the trinocular head and camera side port on the body; beam splitting on the trinocular head – 80:20 beam splitting on the body – 100:0/0:100
• Reflected light illuminator: 100W mercury lamp of a wide wavelength spectrum
• Fluorescence filters: ultraviolet (UV) violet (V) blue (B) green (G)
• Phase-contrast turret condenser for quick and convenient changing of the microscopy technique
• Transmitted light illuminator – 30W halogen bulb providing bright natural light
• Wide range of compatible optional accessories

The kit includes:

• Base with a power input transmitted light source and condenser focusing mechanism stage and revolving nosepiece
• Fluorescence units
• Mercury lamphouse
• Trinocular head
• Infinity plan achromatic objective: PLL 10x/0.25 WD 4.3mm
• Infinity plan achromatic objective: PLL 20х/0.40 WD 8.0mm
• Infinity plan achromatic objective: PLL 40х/0.60 WD 3.5mm
• Infinity plan achromatic objective: PLL 10x/0.25 PHP2 WD 4.3mm
• Infinity plan achromatic objective: PLL 20x/0.40 PHP2 WD 8.0mm
• Infinity plan achromatic objective: PLL 40x/0.60 PHP2 WD 3.5mm
• Eyepiece 10x/22mm with long eye relief (2 pcs.)
• UV shield
• C-mount adapter 1x
• Hex key wrench
• Mercury lamphouse power supply
• Power cord
• Reflected light illuminator power cord
• Dust cover
• User manual and warranty card

Available on request:

• 10x/22mm eyepiece with a scale
• 12.5x/14mm eyepiece (2 pcs.)
• 15x/15mm eyepiece (2 pcs.)
• 20x/12mm eyepiece (2 pcs.)
• 25x/9mm eyepiece (2 pcs.)
• Digital camera
• Calibration slide