MAGUS Lum D400 LCD Fluorescence Digital Microscope

The microscope is designed for studying specimens using the fluorescence microscopy technique in reflected light and brightfield technique in transmitted light. The additional equipment allows for darkfield polarization and phase contrast microscopy. Fluorescence microscopy is based on the ability of some substances to glow when exposed to the light of a certain part of the spectrum. The specimens are exposed to invisible short-wave ultraviolet light or violet blue and green light. The wavelength of the emitted light will be longer than the wavelength of the excitation light. The specimen glows blue cyan green-yellow or red light respectively. Some specimens glow by themselves while others glow after treatment with fluorochromes. The fluorescent microscope is used to study pathogens of tuberculosis chlamydia rabies herpes and other diseases. The microscope can also be used for DNA and chromosome analysis bone marrow and blood smears forensic and pharmacological examination veterinary control studies and sanitary and epidemiological inspection.

Digital camera

MAGUS CHD40 is a digital HDMI camera with three video interfaces and automatic switching between 4K and Full HD depending on the monitor resolution. The camera is equipped with an 8MP sensor and produces realistic images in 4K resolution (3840x2160px) when connected via HDMI or USB3.0. When connected via Wi-Fi the image quality is Full HD (1920x1080px). The camera uses an HDMI interface to connect to a TV monitor or projector directly. In this mode the camera operates autonomously without a connection to a PC. The HDMI interface provides a high and stable transfer rate from the camera to the external screen. You can connect the camera to a PC via Wi-Fi or USB3.0. Video is recorded at 30fps. The camera combines high FPS and high bandwidth HDMI. Therefore videos are vivid with no freezes or gaps between frames. At maximum resolution the image is well-detailed moving objects are visible without bugs and object movement is displayed without delays.

Monitor

The MAGUS MCD40 Monitor is designed to use a visualization system of the MAGUS microscope. It is connected to a camera mounted on the microscope to display real-time images. It supports MAGUS 4K HDMI cameras. The screen diagonal is 13.3 inches. The IPS matrix provides bright images with large viewing angles. If you look at the display at an angle the color reproduction is not distorted. The display can be placed on a folding stand on a table or shelf or mounted directly to the camera or microscope stand.

Microscope head

Trinocular head with infinity-corrected optics. Eyepiece tubes can rotate 360°. The user can adjust eye relief on the eyepiece to fit their height. The digital camera is mounted in the trinocular tube.

Revolving nosepiece

Five objectives. A free slot is used for centering the reflected light source. An additional objective can also be installed in the free slot in order to achieve extra magnification. The revolving nosepiece with objectives is oriented toward the interior – the user can see the objective inserted into the optical path and the space above the stage is free.

Objectives

Infinity plan achromatic objectives and infinity plan fluorite objectives. Standard configuration includes 4x 10x and 100x objectives for brightfield microscopy and a 40x objective for fluorescence microscopy.

Focusing mechanism

Coaxial coarse and fine focusing knobs are located at the base of the microscope on both sides. The user can place their hands on the table and take a relaxed pose while observing. The focusing adjustment is smooth and effortless. On the right there is a coarse focusing lock knob for quick adjustments after changing the specimen. On the left there is a coarse tension adjustment ring for further adjustment.

Stage

The stage does not have an X-axis positioning rack which ensures its ergonomic operation. The mechanical stage with a belt-driven mechanism allows for the smooth movement of the object. The slide holder is fixed with two screws and can be removed for easy operation. For reflected light observations a black stage plate should be placed on the stage to remove stray light.

Reflected light illuminator

The fluorescence excitation source is a 100W mercury lamp. It is extremely bright and has a wide spectrum of wavelengths. The mercury lamp has discrete peaks allowing you to work with many fluorochromes. The reflected light illuminator contains four excitation filters: ultraviolet (UV) violet (V) blue (B) and green (G). The mercury lamp is located in the lamphouse and can be centered in three planes. The heat radiation is conducted away from the lamphouse so that the lamp does not overheat. There is a mount that allows for safe and quick lamp replacement.

Köhler illumination in reflected and transmitted light

Setting up the Köhler illumination enhances the image quality of a specimen. With such illumination you can achieve maximum resolution on each objective and uniform illumination of the field of view with no darkening at the edges. The object of study is in sharp focus and the image artifacts are removed.

Condenser

The condenser has a slot for a darkfield slider or a phase contrast slider. The usage of slider saves time when switching between microscopy techniques in transmitted light.

Transmitted light source

The transmitted light illuminator has a 30W halogen bulb. Halogen bulbs emit light with a color temperature that allows for comfortable work. The 30W bulb is bright enough to observe using 4x to 100x magnification objectives.

Accessories

There is a line of accessories designed for the microscope. Optional objectives provide additional magnification. The optional 10x/0.35 fluorite objective is used for capturing an image of a specimen with a resolution of 0.5μm over a large field of view. Eyepieces that extend the magnification range of the microscope. Optional eyepieces help you maximize the potential of the objective you use most often. A phase contrast device a darkfield condenser and a polarization device offer more microscopy techniques so that you can study the specimens that are invisible in brightfield or fluorescent light. A digital camera that outputs the microscope image on a monitor and stores files along with software that takes real-time measurements of specimens. A calibration slide for measuring objects that can be combined with an eyepiece with a scale or the camera software.

Key features of the microscope:

• Study of specimens emitting fluorescence when exposed to excitation light
• Microscopy techniques: fluorescence in reflected light brightfield in transmitted light
• Trinocular head with adjustable eye relief and a vertical tube for mounting a digital camera
• Reflected light illuminator: 100W mercury lamp of a wide wavelength spectrum
• Fluorescence filters: ultraviolet (UV) violet (V) blue (B) green (G)
• Transmitted light illuminator: 30W halogen bulb providing bright natural light
• Köhler illumination in reflected and transmitted light
• Simple stage without a positioning rack
• Wide range of compatible optional accessories

Key features of the camera:

• The camera operates autonomously without a connection to a PC and via HDMI interface. Can be connected to a PC via Wi-Fi and a USB3.0 interface
• Auto switching between 4K and Full HD depending on monitor resolution
• 30fps for observing moving objects recording video and moving the sample without jerkiness or delays
• SONY Exmor/Starvis color CMOS backlit sensor provides low noise level and high light sensitivity even in low-light conditions. You will get clearer brighter and more color-saturated images
• Software with photo video recording editing external display functions linear and angular measurements

The kit includes:

• MAGUS CHD40 Digital Camera (digital camera HDMI cable (1.5m) USB3.0 cable (1.5m) USB mouse 32GB SD memory card USB Wi-Fi adapter (2pcs.) AC power adapter 12V/1A (Euro) installation CD with drivers and software user manual and warranty card)
• MAGUS MCD40 LCD Monitor
• Base with a power input transmitted light source and condenser focusing mechanism stage and revolving nosepiece
• Reflected light illuminator
• Mercury lamphouse
• Trinocular head
• Infinity plan achromatic objective: PL 4x/0.10 WD 19.8mm
• Infinity plan achromatic objective: PL 10x/0.25 WD 5.0mm
• Infinity plan achromatic objective fluo: PL FL 40x/0.85 (spring-loaded) WD 0.42mm
• Infinity plan achromatic objective fluo: PL 100x/1.25 (spring-loaded oil) WD 0.36mm
• Eyepiece 10x/22mm with long eye relief (2 pcs.)
• UV shield
• C-mount adapter 1x
• Hex key wrench
• Mercury lamphouse power supply
• Power cord
• Reflected light illuminator power cord
• Dust cover
• User manual and warranty card

Available on request:

• 10x/22mm eyepiece with a scale
• 12.5x/14mm eyepiece (2 pcs.)
• 15x/15mm eyepiece (2 pcs.)
• 20x/12mm eyepiece (2 pcs.)
• 25x/9mm eyepiece (2 pcs.)
• Infinity plan achromatic objective fluo: PL FL 10x/0.35 WD 2.37mm
• Infinity plan achromatic objective: PL 60x/0.80 ∞/0.17 WD 0.46mm
• Phase contrast device
• Darkfield condenser
• Immersion darkfield condenser
• Darkfield slider
• Polarization device
• Calibration slide