MAGUS SPH360 Phase Contrast Device with set of Plan S-Apo Ph 4х/10х/20х/40х/60х H60mm objectives

The MAGUS SPH360 phase contrast device consists of phase contrast sliders and a set of phase objectives. The phase contrast device is used to study transparent low-contrast specimens that are not visible in the bright field. Staining kills living cells. The main advantage of the technique is that it may be used to study living unstained organisms in their natural state. The technique is applied in medicine ecology pharmacology agriculture etc.

Phase contrast sliders

The phase contrast slider is installed in the condenser slot. The slider is a plate with round holes: A free hole is for brightfield observations; the other holes have phase inserts that match the objectives being used. The phase contrast device includes two sliders: One has an insert that operates in tandem with 10x 20x and 40x objectives; the other insert operates in tandem with 4х and 60x objectives. The marking of the insert matches the objective being used. The slider allows you to easily switch from the brightfield observations to the phase contrast microscopy and back again. The slider annular diaphragms are aligned with the objective phase rings using a centering telescope supplied with the microscope.

Plan semi-apochromatic phase objectives

Phase objectives differ from regular objectives by having a phase ring in the plane of the exit pupil. Plan semi-apochromatic objectives have chromatic aberrations that are corrected for four colors spherical aberration corrected for three colors and field curvature corrected for 90% of the field of view diameter. The 4x and 10x objectives are used to work with dishes with a bottom thickness of 1.2mm. The 20x 40x and 60x objectives have a correction ring. Rotation of the ring changes the distance between the inner lenses of the objective thereby adjusting the objective optics to work with dishes with various thickness of the bottom. The 20x and 40x objectives are used to study samples in dishes with a bottom thickness from 0mm to 2mm and the 60x objective – in dishes with a bottom thickness from 0.1mm to 1.3mm. The objectives are designed for the infinity-corrected tube length. The parfocal distance is 60mm.

Compatibility:

• MAGUS Bio V360 Biological Inverted Microscope

Package:

• Phase contrast slider for 10х 20х and 40х objectives with centerable rings
• Phase contrast slider for 4х and 60х objectives with centerable rings
• Infinity plan semi-apochromatic phase objective: PlanF S-Apo 4x/0.13 Ph ∞/1.2 WD 17.78mm parfocal distance: 60mm
• Infinity plan semi-apochromatic phase objective: PlanF S-Apo 10x/0.30 Ph ∞/1.2 WD 7.4mm parfocal distance: 60mm
• Infinity plan semi-apochromatic phase objective: PlanF S-Apo 20x/0.45 Ph ∞/ 0–2 WD 7.5–8.8mm parfocal distance: 60mm
• Infinity plan semi-apochromatic phase objective: PlanF S-Apo 40x/0.60 Ph ∞/ 0–2 WD 3–4.4mm parfocal distance: 60mm
• Infinity plan semi-apochromatic phase objective: PlanF S-Apo 60x/1.70 Ph ∞/ 0.1–1.3 WD 1.8–2.6mm parfocal distance: 60mm