MAGUS Lum 490L Fluorescence Microscope

A research-grade microscope for studying specimens in reflected light using fluorescence and in transmitted light using brightfield. Installation of additional accessories will allow for darkfield, phase contrast, differential interference contrast, and polarization techniques. The motorized revolving nosepiece and condenser automate the process of switching objectives and adjusting the lighting system for each of them. The user is not distracted by the settings and therefore focused on the research, which accelerates the speed of operations. Fluorescence microscopy is based on the ability of some substances to glow when exposed to light of a certain part of the spectrum. The reflected light illuminator contains four fluorescence filter cubes that provide optimal conditions for exciting fluorescence in the blue, green, ultraviolet, and red emission wavelength ranges. The microscope is used in medical institutions and laboratories, research organizations, forensic centers, industrial and environmental organizations, pharmaceutical companies, and educational institutions.

Microscope head

Trinocular head with infinity-corrected optics. The user selects the most comfortable angle of inclination of the microscope head in the range of 0° to 35°. The digital camera is mounted on the vertical tube. The beam splits 0/100, 100/0, or 80/20. The basic configuration includes 10x/25mm eyepieces with diopter adjustment and high eyepoint for working with glasses.

Revolving nosepiece

A motorized revolving nosepiece for 6 objectives is oriented away from the observer, toward the stand: The user sees the objective inserted into the optical path, and the space above the stage is free. An additional objective can also be installed in the free slot in order to achieve extra magnification. Above the nosepiece is a slot with a plug for installing an analyzer or differential interference contrast sliders.

Plan semi-apochromatic fluorescence objectives

The objective lenses are made of fluorite, a mineral with a high degree of transparency over a wide wavelength spectrum, without autofluorescence and birefringence, with corrected secondary spectrum. The high numerical aperture enhances the objective's resolving power. The image in the eyepieces and through the digital camera is cleaner, has more contrast, and is brighter than with regular plan achromatic objectives. Plan semi-apochromatic objectives have chromatic aberrations that are corrected for four colors, spherical aberration corrected for three colors, and field curvature corrected for 90% of the field of view diameter. The 4x objective is designed to work with specimens with or without a coverslip. The 10x, 20x, 40x, and 100x objectives are used to observe specimens with a 0.17mm coverslip. The 100x objective supports oil immersion. All of the objectives are designed for the infinity-corrected tube length. The parfocal distance is 60mm.

Remote control of the revolving nosepiece

The front panel of the microscope has objective selection buttons. When you press a button, the revolving nosepiece automatically sets the selected objective to its operating position. A separate button is used to switch between the two most commonly used objectives. On both sides of the body, next to the focusing knobs, there are buttons for rotating the nosepiece. The lighting and the position of the condenser's front lens are automatically adjusted to match the selected objective. These features improve user comfort, speed, and efficiency.

Maintaining a comfortable brightness level in transmitted light when changing magnifications

The objectives of different magnifications transmit light with different levels of intensity, and so each time you change objectives, the brightness of the light must be adjusted. Switching from a higher to a lower magnification objective causes eye fatigue, as the image brightness in the eyepieces increases sharply. The MAGUS Lum 490L, which is equipped with intelligent brightness control, solves this problem. The microscope remembers the brightness for each objective that the user has selected and automatically sets this brightness when turning the nosepiece. Intelligent control reduces the time required to adjust brightness. MAGUS Lum 490L increases user comfort and saves you time even when your work requires frequent magnification changes.

Focusing mechanism

The microscope features low-position coaxial coarse and fine focusing knobs. The researcher can place their hands on the table and take a comfortable position in front of the microscope. The coarse focus lock knob helps you quickly adjust the microscope after changing the object of study. The knob is located on the left side of the microscope on the same axis as the focusing mechanism. The ring on the right side adjusts the tension of the coarse focusing travel. The user adjusts the comfortable tension for work.

Stage

The stage plate is made of sapphire glass. The hardness, durability as well as scratch and chemical resistance of the sapphire glass is second only to diamond glass. It significantly outperforms the popular chemically tempered Gorilla Glass. According to the Mohs scale (a ten-point scale of relative hardness of mineral surfaces), Gorilla Glass scores 6.8, sapphire scores 9, and diamond has a score of 10. The specimen holder is secured with two screws and can be easily removed during manual scanning. The height-adjustable stage control knob ensures user comfort, allowing the user’s hand to rest on the desk for fatigue-free operation. The control knob also features tension adjustment, which allows you to select the optimal rotation tension for smooth and precise specimen movement.

Epi-fluorescence illuminator

There is a 5-position rotating turret inside the attachment with four fluorescence filters of different wavelengths: broadband blue (В), green (G), ultraviolet (UV), and narrowband red (R). The filter is selected by rotating the ring. One position of the turret corresponds to the brightfield observations. An adjustable field diaphragm limits the illuminated field. The excitation light sources are LEDs. The LEDs instantly reach the brightness that is set by the user. They do not heat up during prolonged use and can switch off quickly, if necessary. This makes it easy to switch from the brightfield technique to fluorescence and back again. The LED lifetime is 50,000 hours. It is recommended to choose LED-based fluorescence for higher education as it is the safest option and requires minimal maintenance.

Condenser

Optimal condenser settings for each objective: The flip-down lens automatically moves out of the beam path for low-magnification objectives and returns back for objectives with magnifications greater than 10x. One less manual step – for fast and convenient operation. The user can also individually adjust the position of the flip-down lens for specific studies. The condenser is height-adjustable and can be centered. Mounting type: dovetail. The condenser ring adjusts the iris aperture diaphragm. The condenser body has white aperture markings and color markings for the objective magnification, and the ring has an indicator marker. To achieve a high-contrast image, it is recommended to set the aperture diaphragm slightly below the value corresponding to the objective that is in use.

Transmitted light source

The transmitted light illuminator uses a 3W LED. The color temperature does not change when you adjust the brightness. The LED lifetime is 50,000 hours.

Köhler illumination in transmitted light

The light source is located at the rear of the microscope, and the light is directed to the collector using a mirror. The image contrast is controlled by the condenser aperture diaphragm, while the illuminated field area is adjusted by the field diaphragm. The field aperture control is located on the right at the bottom of the body, next to the focus adjustment area. This simplifies the Köhler illumination setup. The Köhler illumination improves the image quality of the observed sample: Each objective achieves maximum resolution and the field of view is illuminated evenly without darkening at the edges. The specimen is brought into focus, stray light is eliminated, and contrast is enhanced.

Ergonomic design

Physical discomfort causes fatigue and reduces productivity. The ergonomic design of the microscope plays an important role in everyday scientific research. MAGUS Lum 490L provides user comfort during operation. Motorized components increase speed and simplify repetitive tasks when changing objectives frequently. The adjustable head allows the user to select a comfortable viewing angle, thereby reducing neck and back strain. Focusing knobs are located at the bottom of the body. The user does not need to strain their hands. Thanks to the smooth movement of the mechanism, the user can effortlessly focus on the object. The microscope is operated with minimal hand movement, since the long stage control handle and fine focusing knob are located in the same working area.

Accessories

There is a line of accessories designed specifically for this microscope. Optional objectives provide additional magnification. Eyepieces extend the magnification range of the microscope. Optional eyepieces help you maximize the potential of the objective that you use most often. The optional attachments for phase contrast, darkfield, polarization, and DIC expand the range of microscopy techniques, allowing you to study specimens that are invisible in brightfield. Digital cameras output the microscope image to a monitor, store files, and software takes real-time measurements of specimens. A calibration slide is used to measure objects and can be combined with the eyepiece with a scale or with the camera software.

Alternative models

MAGUS Lum 490L can be considered as an alternative to the following models: Carl Zeiss Axiscope 5, Leica DM 2500, Leica DM 2500LED, Leica DM 3000, Leica DM 3000LED, Nikon ECLIPSE Ci, Nikon ECLIPSE Ni, Nikon ECLIPSE Si, Olympus BX 53, Olympus BX43 BX53 Fluo Evident.

Main features:

• Study of specimens emitting fluorescence when exposed to excitation light
• Microscopy techniques: fluorescence in reflected light, brightfield in transmitted light
• Trinocular head with a vertical camera port, adjustable tilt angle, and beam splitting ratio: 0/100, 100/0, or 80/20
• Motorized revolving nosepiece controlled by ergonomic buttons on the lower stand for one-touch objective switching
• Plan semi-apochromatic fluorescence objectives with high resolving power
• Transmitted light illuminator: energy-saving 3W LED with a lifetime of up to 50,000 hours
• Köhler illumination and motorized condenser with a flip‑down lens
• Intelligent lighting control system in transmitted light: automatic brightness selection when changing the working objective
• The stage has a sapphire glass plate and a long control handle for comfort during use
• Wide range of compatible optional accessories

Package:

• Stand with a focusing mechanism, eco-mode support, semi-automatic control of the revolving nosepiece, and a condenser
• Transmitted light LED lamphouse (mounted on the stand)
• Motorized revolving nosepiece (mounted on the stand)
• Stage holder with a condenser mount
• Stage with specimen holder
• Motorized Abbe condenser with flip-down lens
• Epi-fluorescence illuminator
• B/G/UV/R fluorescence filter cubes
• Trinocular microscope head
• Plan semi-apochromatic fluorescence objective PlanF S-Apo 4x/0.13 ∞/– WD 17mm, parfocal distance: 60mm
• Plan semi-apochromatic fluorescence objective PlanF S-Apo 10x/0.30 ∞/0.17 WD 16mm, parfocal distance: 60mm
• Plan semi-apochromatic fluorescence objective PlanF S-Apo 20x/0.50 ∞/0.17 WD 2.7mm, parfocal distance: 60mm
• Plan semi-apochromatic fluorescence objective PlanF S-Apo 40x/0.75 ∞/0.17 WD 1.4mm, parfocal distance: 60mm
• Plan semi-apochromatic fluorescence objective PlanF S-Apo 100x/1.30 oil ∞/0.17 WD 0.18mm, parfocal distance: 60mm
• 10x/25mm eyepiece with high eyepoint and diopter adjustment (2 pcs.)
• Eyecup (2 pcs.)
• 1х C-mount adapter
• Microscope power adapter and power cord
• Power adapter and power cord for the epi‑fluorescence attachment
• Dust cover
• User manual and warranty card

Available on request:

• Plan achromatic objective Plan 2х/0.06 ∞/–, parfocal distance: 60mm
• Plan achromatic objective Plan 60х/0.80 ∞/0.17, parfocal distance: 60mm
• Plan apochromatic objective Plan Apo 60x/1.42 oil ∞/0.17, parfocal distance: 60mm
• Plan achromatic objective Plan 100x/1.10 water ∞/0.17, parfocal distance: 60mm
• 10х/22mm eyepiece with a scale
• 10x/22mm eyepiece with a reticle
• 10x/22mm eyepiece with crosshairs
• 12.5x/16mm eyepiece (2 pcs.)
• 15х/16mm eyepiece (2 pcs.)
• 20х/12mm eyepiece (2 pcs.)
• 30х/8mm eyepiece (2 pcs.)
• Phase contrast device: phase contrast condenser, set of phase objectives, centering telescope
• Darkfield condenser NA 0.7–0.9
• Darkfield immersion condenser NA 1.3–1.26
• Differential Interference Contrast (DIC) device
• Epi-fluorescence attachment featuring a mercury lamphouse, fluorescence filter cubes, and a mercury lamp power supply unit
• Polarizer/analyzer set
• Digital camera
• Calibration slide
• Monitor
• Set of color filters (blue, green, yellow, frosted glass)