MAGUS Lum 450L Fluorescence Microscope

The microscope is designed for studying specimens using the fluorescence microscopy technique in reflected light and the brightfield technique in transmitted light. Additional equipment allows for darkfield polarization and phase contrast.
The coded revolving nosepiece maintains a comfortable brightness level when switching objectives. Intelligent control of microscope lighting increases the comfort and speed of the researcher’s routine work. “Smart” functions help students more easily enter the profession and gain the necessary professional experience.
The fluorescence microscopy is based on the ability of some substances to glow when exposed to light of a certain part of the spectrum. With the MAGUS Lum 450L objects are irradiated with blue and green light. The wavelength of the emitted light will be longer than the wavelength of the excitation light. The specimen glows with green-yellow or red light respectively. Some specimens glow by themselves while others glow after treatment with fluorochromes. The fluorescent microscope is used to study the pathogens of tuberculosis chlamydia rabies and herpes as well as other diseases. The microscope can be used for DNA and chromosome analysis bone marrow and blood smears forensic and pharmacological examination veterinary control studies and sanitary and epidemiological inspection.

Microscope head

Trinocular head with infinity-corrected optics. Eyepiece tubes rotate 360°. The user can adjust the eye relief on the eyepiece to fit their height. The digital camera is mounted in the trinocular tube. The beam splitting is 50/50.
The base kit includes 10x/22mm eyepieces with diopter adjustment and a long eye relief for working with glasses. Flat rubber eyecups protect the optics from scratches.

Revolving nosepiece and objectives

The coded revolver for 5 objectives is oriented toward the interior: The user can see the objective inserted into the optical path and the space above the stage is free. An additional objective can also be installed in the free slot in order to achieve extra magnification. The parfocal distance is 60mm.
Above the revolver there is a slot with a plug for installing the analyzer.

Maintaining comfortable brightness levels when switching magnifications

The objectives of different magnifications transmit light with different intensity and so each time you change objectives the brightness of the light must be adjusted. In addition brightness increases sharply when switching from a higher magnification objective to a lower magnification one. A sharp increase in brightness causes eye fatigue. MAGUS Lum 450L is equipped with smart brightness control. The microscope remembers the brightness for each objective that the user has selected and automatically sets this brightness when turning the nosepiece. Intelligent control reduces the time required to adjust the brightness. MAGUS Lum 450L increases user comfort and saves time even when work requires frequent magnification changes.

Focusing mechanism

Coaxial coarse and fine focusing knobs.
The coarse focusing knob is located on the left side. Fine focusing knobs are located on both sides; the right knob has recesses for fingers.
The coarse focus lock knob helps you quickly adjust the microscope after changing the subject of study. The knob is located on the left side of the microscope on the same axis as the focusing mechanism.
The ring on the right side adjusts the tension of the coarse focusing travel. The user adjusts the comfortable tension for work.

Stage

To ensure the ergonomic operation of the stage it does not have an X-axis positioning rack. The belt-driven mechanism allows for the smooth movement of the specimen. The specimen holder is secured with two screws and can be easily removed during manual scanning.
The long stage control handle ensures user comfort while working: Your hand can rest on the table without strain.

Reflected light illuminator

Light sources for the excitation of luminescence are 3W LEDs along with filters of two wavelength ranges of luminescence: blue (B) and green (G). Setting the switch on the reflected light illuminator to the left or right position changes the working filter. The central position of the switch corresponds to the brightfield technique.
The LEDs will instantaneously reach full brightness won’t heat up during prolonged use and can turn off quickly. That makes it easy to switch from the brightfield technique to fluorescence and back again. The lifetime of the LEDs is 50 000 hours. It is recommended to choose an LED-based fluorescence microscope for educational purposes as the safest and low-maintenance option.

Köhler illumination in transmitted light

Setting up the Köhler illumination enhances the image quality of a specimen. With such illumination you achieve maximum resolution on each objective. Even illumination of the field of view with no darkening at the edges. The object of study is in sharp focus and the image artifacts are removed.

Condenser

The condenser is height-adjustable and can be centered. Mounting type: dovetail.
The condenser ring adjusts the iris aperture diaphragm. The condenser body has a marking for the magnification of the objectives and the ring has an indicator marker. To achieve contrast on each objective it is recommended to rotate the ring so that the index marker matches the magnification marking of the objective being used.
The condenser has a slot with a plug for a darkfield slider or a phase contrast slider. The installation of a slider saves time when changing the observation method.

Transmitted light source

The transmitted light illuminator has a 3W LED. The color temperature does not change when adjusting the brightness. The LED has a lifespan of 50 000 hours.

LCD status screen

The LCD screen on the base of the microscope displays the objective magnification brightness and color temperature of the light source as well as operating mode (“sleep” “eco”).
Using the screen and two knobs the microscope user adjusts the brightness locks brightness adjustment and sets the sleep mode and the auto-off timer.

Ergonomic design

Physical discomfort causes fatigue and reduces productivity. The ergonomic design of the microscope plays an important role in everyday scientific research. The MAGUS Lum 450L provides user comfort during work.
By rotating the microscope head the user selects a point of view height so as not to strain their back and neck.
Focusing knobs are located at the bottom of the body. The user does not strain their hands. Thanks to the smooth movement of the mechanism the user can effortlessly focus on the object.
The microscope is operated with minimal hand movement since the long stage control handle and fine focusing knob are located in the same working area. The microscope is equipped with a special carrying handle.
The design for the hidden placement of the power cord improves the workplace esthetic and safety of carrying the microscope as well as simplifies the storage of the device.

Accessories

There is a line of accessories designed for this microscope.
Optional objectives provide additional magnification.
The eyepieces extend the magnification range of the microscope. Optional eyepieces help you maximize the potential of the objective you use most often.
The phase contrast device darkfield condenser and polarization device offer more contrast techniques and so you can study the specimens that are invisible in brightfield.
A digital camera outputs the microscope image to a monitor store files and software takes real-time measurements of specimens.
A calibration slide is used to measure objects and it can be combined with the eyepiece with a scale or with the camera software.

Key features:

• Study of specimens emitting fluorescence when exposed to excitation light
• Microscopy techniques: fluorescence in reflected light brightfield in transmitted light
• The trinocular head with a vertical tube for installing a digital camera and height adjustment to adjust to the observer; the light beam splitting is 50/50
• Coded revolving nosepiece: The brightness of the light source is set automatically depending on the selected objective
• Reflected light illuminator – 3W LEDs (2 pcs.) of different wavelengths
• Visible luminescence excitation filters: blue (B) green (G)
• Transmitted light illuminator: energy-saving 3W LED with a lifetime of up to 50 000 hours
• Köhler illumination in transmitted light
• Centerable and height-adjustable condenser with objective magnification markings
• Smart lighting control system: automatic brightness selection dimming lock timer auto-off LCD operating status screen
• Stage without an X-axis positioning rack and with a long control handle for convenient operation
• Ergonomic stand with a carrying handle and the hidden placement of the power cord
• Wide range of compatible optional accessories

The kit includes:

• Stand with built-in power supply reflected visible light illuminator transmitted light source focusing mechanism stage condenser mount and revolving nosepiece
• Abbe condenser
• Reflected light illuminator
• Trinocular head
• Plan achromatic infinity-corrected objective: 4x/0.10 parfocal height 60mm
• Plan achromatic infinity-corrected objective: 10x/0.25 parfocal height 60mm
• Plan achromatic infinity-corrected objective: 40x/0.65 (spring-loaded) parfocal height 60mm
• Plan achromatic infinity-corrected objective: 100x/0.25 oil (spring-loaded) parfocal height 60mm
• Eyepiece 10x/22 mm with long eye relief and diopter adjustment (2 pcs.)
• Eyepiece eyecup (2 pcs.)
• Light filter
• C-mount camera adapter
• Bottle of immersion oil
• AC power cord
• Dust cover
• User manual and warranty card

Available on request:

• Eyepiece 10x/22mm with scale
• Eyepiece 10x/22 mm with reticle
• Eyepiece 10x/22mm with crosshairs
• 12.5x/17.5mm eyepiece (2 pcs.)
• 15x/16mm eyepiece (2 pcs.)
• 20x/12mm eyepiece (2 pcs.)
• Plan achromatic infinity-corrected objective: 20x/0.40 parfocal height 60mm
• Phase contrast device: set of phase sliders auxiliary centering telescope set of phase objectives
• Darkfield slider
• Polarization device
• Digital camera
• Calibration slide
• Monitor