MAGUS SPH2 Phase Сontrast Device

The MAGUS SPH2 phase contrast device consists of a set of phase contrast sliders a set of phase objectives and a centering telescope. The phase contrast device is used to study transparent low-contrast specimens that are not visible in the bright field. Staining kills living cells. The main advantage of the technique is that it may be used to study living unstained organisms in their natural state. The technique is applied in medicine ecology pharmacology agriculture etc.

Phase contrast sliders

The phase contrast slider is installed in the condenser slot. The slider is a plate with two holes: A free hole for brightfield observations; the second hole has a phase insert that matches the objective being used. The phase contrast device includes two sliders: one has an insert that operates in tandem with 10x 20x and 40x objectives; the other insert operates in tandem with a 100x objective. The marking of the insert matches the objective being used. The slider allows you to easily switch from brightfield observations to the phase contrast technique and back again.

Plan achromatic phase objectives

Phase objectives differ from regular objectives by having a phase ring in the plane of the exit pupil. Plan achromatic phase objectives form an image that has the same focus in the center and at the edges and therefore the distortion is negligible. Chromatic aberrations are corrected for blue and red spherical aberration – for green and the field curvature is corrected for 90% of the field of view diameter. The 10x objective is designed to work with specimens with or without a coverslip. The 20x 40x and 100x objectives are used to observe specimens with a 0.17mm coverslip. The 100x objective supports oil immersion. The objectives are designed for the infinity-corrected tube length. The parfocal distance is 60mm.

Centering telescope

Using a centering telescope the slider annular diaphragms are aligned with the objective phase rings.

Compatibility:

• MAGUS Bio 260T Biological Microscope
• MAGUS Bio DH260 Biological Digital Microscope

Package:

• Phase contrast slider for 10х 20х and 40х objectives with centerable rings
• Phase contrast slider for 100х objective with centerable rings
• Infinity plan achromatic phase objective Plan 10x/0.25 Ph ∞/- WD 10.2mm parfocal distance: 60mm
• Infinity plan achromatic phase objective Plan 20x/0.40 Ph ∞/0.17 WD 4.8mm parfocal distance: 60mm
• Infinity plan achromatic phase objective Plan 40x/0.65 Ph ∞/0.17 WD 1.5mm parfocal distance: 60mm
• Infinity plan achromatic phase objective Plan 100x/1.25 oil Ph ∞/0.17 WD 0.22mm parfocal distance: 60mm
• Centering telescope