MAGUS 10PL60V Plan 10х/0.25 ∞/1.2 H60mm Objective

Objective for brightfield observations. The MAGUS Bio V360 microscope kit includes phase objectives. The inner lens of such an objective has a metal film ring etched on it which is required for phase-contrast observations. The metal film provides a quarter-wavelength phase shift relative to the rest of the light and it reduces light transmission. Therefore objectives without phase plates are recommended for brightfield observations.

The resolution limit of a 10х/0.25 objective is 1.1μm. Chromatic aberrations are corrected for blue and red spherical aberration for green and the field curvature is corrected for 90% of the field of view diameter.

The objective is specifically designed for the infinity-corrected tube length. The parfocal distance is 60mm. When a 10х/22 mm eyepiece is used the diameter of the field of view is 2.2mm. The objective is for working with dishes with a bottom thickness of 1.2mm.

The lower limit of the microscope’s useful magnification when a 10x objective is used is 125x. Lower magnification does not allow you to distinguish the details of the specimen whose image is formed by the objective. The upper limit of the useful magnification when a 10x objective is used is 250x. Higher magnification is considered “useless” as it provides no new detail in the image of the sample. When a 10x/0.25 objective is used use 12.5x–25x eyepieces to utilize the useful magnification of the microscope and 10x eyepieces to scan the specimen and search the area of the sample you want to observe.

Plan achromatic objectives form an image that has the same focus in the center and at the edges and therefore the distortion is negligible. This strength is used for digital imaging and combining them with wide-field eyepieces with a 22mm field of view.

Compatibility:

• MAGUS Bio V360 Biological Inverted Microscope