MAGUS Lum D460L Fluorescence Digital Microscope

Microscope is designed for observing specimens in reflected light using the fluorescence technique and in transmitted light using the brightfield technique. With optional accessories, you can also use darkfield, simple polarization, and phase-contrast techniques. The coded revolving nosepiece maintains a comfortable brightness level when the objectives are changed. The microscope's intelligent lighting control system improves comfort and speed of the researcher’s everyday work. Smart features help students ease into the profession and gain the professional experience they need. The fluorescence technique is based on the ability of substances to emit the light when excited by the light of a certain wavelength. On the MAGUS Lum D460L, specimens are excited with blue, green, and ultraviolet light. The wavelength of the emitted light is longer than the wavelength of the excitation light. Some specimens exhibit autofluorescence, while others should be treated with fluorochromes. The microscope is equipped with a 1.7MP camera with a USB 3.0 interface. The camera connects to a computer and displays the image on its screen. The software complements the system with analysis and documentation functions. The microscope is used in medical institutions and laboratories, research organizations, forensic centers, industrial and environmental organizations, pharmaceutical companies, and educational institutions.

Digital camera

The MAGUS CLM50 digital camera is designed for use in fluorescence and darkfield microscopy techniques. The camera is equipped with a 1.7MP sensor producing a realistic image in 1600x1100px. Video is recorded at 33fps at maximum resolution. Videos are smooth, with soft and subtle transitions between frames. The movement of the sample is displayed in real time with no delays. The camera provides convenient work with moving objects and is suitable for conducting demonstrations in a classroom. The camera is equipped with a USB 3.0 interface. The data transfer rate is 10 times faster than that of USB 2.0 cameras. The high-speed camera is recommended for professional laboratories, research, or university training.

Microscope head

Trinocular, with infinity-corrected optics. The eyepiece tubes are 360° rotatable. The user can adjust the eyepiece height to suit their individual stature. The digital camera is mounted in the trinocular tube. The beam splitting ratio is fixed at 50/50. The vertical tube provides 0.55x magnification. When paired with the included 1x adapter, the final magnification at the camera is 0.55x. When calculating the field of view and on-screen magnification for each objective, use a total factor of 0.55x. The basic configuration includes 10x/22mm eyepieces with diopter adjustment and high eyepoint for working with glasses. Flat rubber eyecups protect eyeglasses from scratches.

Revolving nosepiece and objectives

The coded revolver for five objectives is oriented toward the interior: The user can see the objective inserted into the optical path, and the space above the stage is free. An additional objective can also be installed in the free slot in order to achieve extra magnification. The parfocal distance is 60mm. The 40x objective with its extended working distance of 1.5mm provides additional protection for the front lens against immersion oil contact when rotating the nosepiece. Above the revolver, there is a slot with a plug for the analyzer.

Maintaining a comfortable brightness level in transmitted light when changing magnifications The objectives of different magnifications transmit light with different levels of intensity, and so each time you change objectives, the brightness of the light must be adjusted. Switching from a higher to a lower magnification objective causes eye fatigue, as the image brightness in the eyepieces increases sharply. The MAGUS Lum D460L, which is equipped with intelligent brightness control, solves this problem. The microscope remembers the brightness for each objective that the user has selected and automatically sets this brightness when turning the nosepiece. Intelligent control reduces the time required to adjust brightness. MAGUS Lum D460L increases user comfort and saves you time even when your work requires frequent magnification changes.

Focusing mechanism

Coaxial coarse & fine focusing knobs. The coarse focusing knob is located on the left side. Fine focusing knobs are located on both sides, the right knob having special finger indentations. The ring on the left side adjusts the tension of the coarse focusing travel. The user adjusts the comfortable tension for work.

Stage

The stage has no X-axis rack and pinion, which improves ergonomics. The belt-driven mechanism allows for smooth movement of the specimen. The specimen holder is secured with two screws and can be easily removed during manual scanning. The long stage control handle ensures user comfort: The hand rests on the table with no strain.

Motorized fluorescence attachment

There is a 4-position rotating turret inside the attachment with three broadband fluorescence filters of different wavelengths: blue (В), green (G), and ultraviolet (UV). The required filter is introduced into the optical path by clicking the button. One button on the attachment rotates the turret to the next filter clockwise, and the other one rotates the turret counterclockwise. One position of the turret corresponds to brightfield observations in transmitted light. The excitation light sources are LEDs. The LEDs instantly reach the brightness that is set by the user. They do not heat up during prolonged use and can switch off quickly, if necessary. This makes it easy to switch from the brightfield technique to fluorescence and back again. The brightness of the LEDs is controlled by two additional buttons that are located in the same work area as the turret switching buttons. The LED lifetime is 50,000 hours. It is recommended to choose LED-based fluorescence for higher education as it is the safest option and requires minimal maintenance.

Köhler illumination in transmitted light

Properly setting up Köhler illumination enhances the image quality of a specimen. With such illumination, you achieve maximum resolution for each objective and an evenly illuminated field of view with no darkening at the edges. The object of study is in sharp focus and the image artifacts are removed.

Condenser

The condenser is height-adjustable and can be centered. Mounting type: dovetail. The condenser ring adjusts the iris aperture diaphragm. The condenser body has a marking for the magnification of the objectives, and the ring has an indicator marker. To achieve contrast on each objective, it is recommended to rotate the ring so that the index marker matches the magnification marking of the objective being used. The condenser has a slot with a plug for a darkfield slider or a phase contrast slider. The installation of a slider saves time when changing the observation method.

Transmitted light source

The transmitted light illuminator uses a 3W LED. The color temperature does not change when you adjust the brightness. The LED lifetime is 50,000 hours.

Status LCD screen

The LCD screen on the base of the microscope displays the objective magnification, brightness of the light source, and operating mode (“sleep” and “eco”). Using the screen and two knobs, the microscope user adjusts the brightness, locks the brightness adjustment, and sets the sleep mode and auto-off timer.

Ergonomic design

Physical discomfort causes fatigue and reduces productivity. The ergonomic design of the microscope plays an important role in everyday scientific research. MAGUS Lum D460L provides user comfort during operation. By rotating the eyepiece tubes, the user can adjust the viewing height to prevent back and neck strain. Focusing knobs are located at the bottom of the body. The user does not need to strain their hands. Thanks to the smooth movement of the mechanism, the user can effortlessly focus on the object. The microscope is operated with minimal hand movement, since the long stage control handle and fine focusing knob are located in the same working area. The microscope is equipped with a special carrying handle. The design for the hidden placement of the power cord improves the workplace esthetic and safety of carrying the microscope as well as simplifies storage of the device.

Accessories

There is a line of accessories designed specifically for this microscope. Optional objectives provide additional magnification. Eyepieces extend the magnification range of the microscope. Optional eyepieces help you maximize the potential of the objective that you use most often. The phase contrast device, darkfield slider, and polarizer/analyzer set offer more contrast techniques so that you can study specimens that are invisible in brightfield. A calibration slide is used to measure objects and can be combined with the eyepiece with a scale or with the camera software.

Alternative models

MAGUS Lum D460L is an alternative for the following models: Carl Zeiss Primostar 3, Leica DM 1000, Leica DM 1000LED, Leica DM 2000, Leica DM 2000LED, Olympus CX 33.

Microscope features:

• Study of specimens emitting fluorescence when exposed to excitation light
• Microscopy techniques: fluorescence in reflected light, brightfield in transmitted light
• Trinocular head with height adjustment to accommodate the observer, a vertical tube for mounting a digital camera, and 50/50 beam splitting
• Coded revolving nosepiece: The brightness of the light source is set automatically depending on the selected objective
• Transmitted light illuminator is energy-saving 3W LED with a lifetime of up to 50,000 hours
• Transmitted Köhler illumination, centered and height-adjustable condenser with objective magnification markings
• Smart transmitted light control system: automatic brightness selection, brightness adjustment lock, auto-off timer, LCD operating status screen
• Stage without an X-axis positioning rack and with a long control handle for convenient operation
• Ergonomic stand with a carrying handle and the hidden placement of the power cord
• Wide range of compatible optional accessories

Camera features:

• For fluorescence and darkfield observations
• 1.7MP resolution for obtaining informative images at high magnification along with low noise and low power dissipation
• 33fps for observing moving objects, recording video, and moving the sample without jerking or delays
• Peltier element reduces the sensor temperature during prolonged operation and eliminates thermal noise. Therefore, long shutter speeds can be used when taking photos
• Global shutter for fast signal readout from the sensor, increased image brightness, and no false optical effects when observing moving objects
• SONY Exmor color CMOS backlit sensor provides a low noise level and high light sensitivity even in low-light conditions. You will get clearer, brighter, and more color-saturated images
• USB 3.0 interface for fast data transfer with no jerking or lagging
• Software with functions for taking photos, recording video, editing, displaying images on an external screen, and measuring linear and angular dimensions

Package:

• MAGUS CLM50 digital camera (digital camera, USB cable, power adapter, carrying case, USB flash drive with drivers and software, user manual, and warranty card)
• Stand with a built-in power supply, transmitted light source, focusing mechanism, stage, condenser mount, and revolving nosepiece
• Abbe condenser
• Motorized fluorescence attachment
• B/G/UV fluorescence filter cubes
• Trinocular microscope head
• Plan achromatic objective Plan 4x/0.10 ∞/0.17, parfocal distance: 60mm
• Plan achromatic objective Plan 10x/0.25 ∞/0.17, parfocal distance: 60mm
• Plan achromatic objective Plan 40x/0.65 ∞/0.17, parfocal distance: 60mm
• Plan achromatic objective Plan 100x/1.25 oil ∞/0.17 (spring-loaded), parfocal distance: 60mm
• 10х/22mm eyepiece with high eyepoint and diopter adjustment (2 pcs.)
• Eyecup (2 pcs.)
• C-mount adapter
• Microscope power cord
• Power adapter for a fluorescence attachment
• Dust cover
• User manual and warranty card

Available on request:

• 10х/22mm eyepiece with a scale
• 10x/22mm eyepiece with a reticle
• 10x/22mm eyepiece with crosshairs
• 12.5х/16mm eyepiece (2 pcs.)
• 15х/16mm eyepiece (2 pcs.)
• 20х/12mm eyepiece (2 pcs.)
• 30х/8mm eyepiece (2 pcs.)
• Plan achromatic objective Plan 2х/0.06 ∞/–, parfocal distance: 60mm
• Plan achromatic objective Plan 20х/0.40 ∞/0.17, parfocal distance: 60mm
• Plan achromatic objective Plan 60х/0.80 ∞/0.17, parfocal distance: 60mm
• Plan apochromatic objective Plan Apo 60х/1.42 oil ∞/0.17, parfocal distance: 60mm
• Plan achromatic objective Plan 100х/1.10 ∞/0.17 water, parfocal distance: 60mm
• Set of plan semi-apochromatic fluorescence objectives: 4x, 10x, 20x, 40x, 100x (oil); ∞/0.17; parfocal distance 60mm
• Phase contrast device: set of phase sliders, auxiliary centering telescope, set of phase objectives
• Phase contrast device: phase contrast condenser, set of phase objectives, auxiliary centering telescope
• Darkfield slider
• Dry darkfield condenser NA 0.7–0.9
• Oil darkfield condenser NA 1.3–1.26
• Polarizer/analyzer set
• Calibration slide
• Set of color filters (blue, green, yellow, frosted glass)
• C-mount adapter
• LCD Monitor and digital camera with an HDMI interface